TeachAde

Embryoid body formation assay

Embryoid body formation assay is a key method for evaluating iPSC pluripotency and germ layer differentiation. By culturing iPSCs in suspension, researchers induce spontaneous aggregation into 3D structures representing ectoderm, mesoderm, and endoderm. Molecular and histological analyses confirm lineage-specific marker expression, supporting studies in regenerative medicine and disease modeling.