TeachAde

C3b deposition assay

C3b deposition assay enables precise quantification of complement activation on cell surfaces. Using ELISA or flow cytometry, this method detects C3b fragments bound to target cells after controlled serum incubation. It supports evaluation of immune evasion, drug efficacy, and pathway-specific complement responses. Suitable for serum, plasma, or cell suspensions, the assay provides reproducible data for autoimmune research, infectious disease studies, and therapeutic development.